| 摘要 |
Disclosed in the present invention are an isolated oligonucleotide and use thereof. The isolated oligonucleotide comprises: a first nucleic acid molecule, the first nucleic acid molecule encoding a double-base recognition module, wherein the double-base recognition module consists of a first single-base recognition module and a second single-base identification module in series, and the first single-base recognition module and the second recognition module both contain repeat variable di-residue amino acids; a first amplification sequence, wherein the first amplification sequence is located at the 5' side of the first nucleic acid molecule, and the first amplification sequence comprises a lis-type endoenzyme recognition sequence; and a second amplification sequence, wherein the second amplification sequence is located at the 3' side of the first nucleic acid molecule, and the second amplification sequence comprises a lis-type endoenzyme recognition sequence. The oligonucleotide can quickly obtain various combinations of RVD, thereby being capable of identifying any target nucleic acid sequence. |
