| 摘要 |
<p>PURPOSE:To keep function of cultivated cell and to promote its multiplication propertis extremely well, by using ovomacroglobulin of egg white protein as an addition agent for cell culture. CONSTITUTION:Ovomacroglobulin, high-molecular-weight glycoprotein in egg white protein, is used as an addition agent for cell culture. Ovomacroglobulin can be prepared by various treatment operations by using its physical and chemical properties. For example, egg white is blended with a water-soluble solvent such as tris-hydrochloric acid buffer solution, insoluble protein components such as ovomucin, etc. are removed, the blend is subjected to gel filtration to give ovomacroglobulin. Since the addition agent, different from serum, is a simple substance, it is not an obstacle in isolation of a reaction product of cell, and provide uniformly a cultivated cell with effects.</p> |
