| 摘要 |
1. Method for preparing a Bacillus strain in the chromosome of which a specified gene M has been amplified, that is to say is present in several copies, characterized in that : a) the integration is preformed, in the chromosome of the said strain, of at least one DNA sequence, referred to as an integration sequence, containing at least : one DNA sequence, referred to as an amplifiable unit, of structure : -D-M-D- containing at least the said gene M and, at each end, two identical sequence D in the direct orientation, these sequences D providing for the integration of the gene M in a non-essential gene of Bacillus ; the amplifiable unit coding, in addition, for a selectable gene ; b) the Bacillus strains obtained by culturing on a selection medium corresponding to the selectable gene are selected, and the strains of which the phenotype corresponds to the presence of an increased copy number of the said gene, compared with the bacterial population before selection, are withdrawn. |
