| 摘要 |
<p>It has been a problem to find an alternative, less time-consuming, and more reliable source of factor IX, a polypeptide which is essential to the human blood-clotting process and necessary for the treatment of patients with Christmas disease. In order to aid in the solution of the problem, there is provided recombinant DNA containing a DNA sequence occurring in the human factor IX genome, and includes recombinant DNA comprising substantially the whole sequence of human factor IX genome, which is inserted in a cloning vehicle and transformed into a host, such as Escherichia coli. Other fragments of the sequence have also been cloned and the invention includes DNA molecules comprising part or all of the human factor IX DNA. There is also described cDNA derived from human factor IX RNA. Uses include the provision of an intermediate of value in the genetic engineering of a factor IX polypeptide precursor and thence manufacture of the factor IX polypeptide, and in making probes for use in diagnosing the presence of normal or abnormal factor IX DNA in patients with Christmas disease.</p> |
