| 摘要 |
PURPOSE:To produce a substance GIF-2 having an ability to cause bacteriostatic action on insect-parasitic microbial group microorganisms in the microbial engineering manner, by using a specific microorganism. CONSTITUTION:A strain having the ability to produce a substance GIF-2, e.g. Bacillus cereus SW (FERM-P No.7242), is used. Thus, the above-mentioned strain is aerobically cultivated in a culture medium, e.g. a culture medium of about 7pH consisting of about 3% polypeptone, about 0.5% yeast extract, about 0.5% NaCl and about 96% deionized water at about 20-40 deg.C for about 2-3 days to collect the aimed substance GIF-2 from the resultant culture filtrate. The above-mentioned substance GIF-2 is white prismatic microcrystals having 1,057mol.wt. and 210-215 deg.C melting point and has an estimated structural formula expressed by formula I (R is beta-amino acid residue expressed by formula II; one of Xs has amide bond). |
