| 摘要 |
PURPOSE:To obtain a novel antibiotic SF-2312 substance having antibacterial action on Gram-negative bacteria, by cultivating an actinomycetes belonging to the genus Micromonospora. CONSTITUTION:An actinomycetes belonging to the genus Micromonospora is cultivated in a medium containing an ordinary nutritive substance. In the cultivation, addition of vitamin B12 promotes growth of mold, and productivity of antibiotic is extremely raised. A mold is removed from the culture by filtration, and a novel antibiotic SF-2312 is obtained from the filtrate by combinations of adsorption, elution, column chromatography, etc. The substance is a water-soluble and acidic antibiotic. Its sodium salt has 21.12% carbon, 3.38% hydrogen, 5.97% nitrogen by weight ratio, 219 molecular weight, apparance of white powder, is water-soluble but slightly soluble or insoluble in methanol, benzene, etc. |
