IMMUNOCHEMICAL ASSAY OF HUMAN CHORIONIC GONADOTROPIN AND REAGENT THEREFOR

摘要

The immunochemical assay of human chorionic gonadotropin (hCG) is disclosed involving the use of an antibody supported on a carrier, an antigen and an antibody labeled with a labeling agent. The supported antibody and the labeled antibody are dissimilar antibodies which are non-overlapping with each other in antigen-determinant position, the supported antibody is an antibody specifically reactive to hCG, and the labeling agent is .beta.-D-galactosidase which is coupled with the corresponding antibody using N,N'-O-phenylene-dimaleimide. The method enables a high reproducibility to hCG to be obtained.