| 摘要 |
<p>The amount of lipid bound sialic acid in a whole blood sample may be determined by a method, which may be automated, involving the following steps: adding to the sample a lower alkyl alcohol and deionized distilled water; mixing the resulting admixture; adding to it a mixture of lower alkyl chlorinated hydrocarbon and a lower alkyl alcohol; treating by mixing and centrifuging the mixture until a recoverable, substantially clear upper phase forms; recovering the upper phase and adding to it a protein-precipitating agent, preferably in admixture with an adsorbing material; mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present.</p><p>The presently preferred mixture for addition to the upper phase is a mixture of 75% by weight phosphotungstic acid and 25% by weight silica gel on a dry weight basis.</p><p>By so determining the amount of lipid bound sialic acid present in a whole blood sample and comparing the amount with values obtained for subjects known to have cancer one may diagnose the presence of cancer in a subject. Alternatively, by determining the amount of lipid bound sialic acid present in whole blood samples obtained from a subject over a period of time and comparing each amount so determined with preceding amounts one may monitor the progression, remission or recurrence of cancer in a subject.</p> |
