发明名称 |
PROCESS FOR PREPARING CDNS CLONES DETERMINING POLYPEPTIDES EXPRESSING CELL GROWTH FACTOR ACTIVITY INFLUENCING PLURAL CELL LINES AND/OR MASTOCYTE GROWTH FACTOR ACTIVITY |
摘要 |
Plasmid vectors are provided that carry complementary DNA (cDNA) clones coding for polypeptides exhibiting mammalian multi-lineage growth cell activity. One of these polypeptides is 166 amino acids in length, including a potential leader sequence of about 19 amino acids. The cDNA is derived from messenger RNA isolated from a mouse T-cell line after activation with concanavalin A. The cDNA was cloned by incorporation into a plasmid vector, which was then transformed into E. coli. The plasmid vector also contains DNA segments from the SV40 virus, permitting expression of the cDNA after transfection into a mammalian host cell, such as monkey COS-7 cells. |
申请公布号 |
HUT36860(A) |
申请公布日期 |
1985.10.28 |
申请号 |
HU19840003742 |
申请日期 |
1984.10.03 |
申请人 |
SCHERING BIOTECH CORP |
发明人 |
YOKOTA,TAKASHI,US;LEE,FRANK,US;RENNICK,DONNA M.,US;ARAI,KEN-ICHI,US |
分类号 |
A61K38/00;C07H21/04;C07K14/52;C12N15/27;C12P21/00 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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