| 摘要 |
A one-step method (and sphering agent/fixing agent useful therein) for treating mammalian red blood cells in a sample which can be effectively measured electrooptically for determination of red blood cell volumes, which comprises treating an anticoagulated whole blood sample with a single aqueous sphering agent/fixing agent reagent at a temperature in the range of 4 DEG C to 37 DEG C over a reaction period of up to 180 seconds employing a whole blood dilution factor of from 1:300 to 1:4,000, wherein said one-step, single reagent treatment provides isovolumetric sphering of the erythrocytes in the sample independent of the concentration of said erythrocytes in the whole blood sample and without inducing lysis of said erythrocytes in the resulting diluted sample. The preferred sphering agent is an alkali metal salt of an alkyl sulfate in an amount 0.5 to 3.0 mg %, and the preferred fixing agent is an aldehyde in an amount from 0.05 to 0.3%. |
