Arrangement for measuring fluorescence polarization

摘要

Arrangement for measuring the fluorescence polarization of cells or particles which are aligned individually along a flow stream illuminated by means of an illumination field wherein the cells are passed in succession through two light beams so as to excite fluorescence; the directions of polarization of the two excitation beams are perpendicular to one another; a pair of detector channels are provided to successively record the two polarization components of the fluorescence at both points of excitation; and an analysis system is provided which calculates the corrected degree of fluorescence for the individual cells from the measured values provided by the detector channels.