| 摘要 |
PURPOSE:To obtain PS antibody sensitized blood cells by conjugating anticandida polysaccharide antibody (PS antibody) via a coupling agent with red blood cells. CONSTITUTION:The red blood cells to be used for sensitized blood cells are preferably fixed red blood cells in terms of preservation and are formed by treating the raw red blood cells suspended by an alsever liquid with gaseous carbon monoxide and adding formalin thereto to fix the cells. A tannic acid, etc. are used for a coupling agent. The bacteria for candida are obtd. by inoculating the same in the Sabouraud's broth, etc. and culturing the same at about 37 deg.C. The anticandida bacterium antibody is obtd. of the bacteria killed by a heating treatment, etc. and are immunized with a rabbit, etc. A polysaccharide antigen is then mixed with the anticandida antibody and the precipitate of only the immune complex is made. The precipitate is centrifugally dispensed and an acetic acid buffer soln. is added thereto, to which a satd. ammonium sulfate soln. is added to settle the antibody. The PS antibody is refined by centrifugal sepn., dialysis, etc. The red blood cells are treated by using the coupling agent and the liquid contg. the PS antibody is brought into contact therewith, by which the PS antibody sensitized red blood cells are obtd. |
