| 摘要 |
<p>A method and apparatus are described for the determination of an analyte in an aqueous liquid which eliminate the biasing effect of unknown interferents which are either preformed or formed in situ during the determination. The method comprises physically contacting a sample of the liquid with an interactive composition for the analyte, measuring the spectrophotometric responses generated by such contact at a primary wavelength λ1 and one or more secondary wavelengths λ2, λ3, ...λn, and determining analyte concentration or activity using the equation:
wherein C is analyte concentration or activity, A,, A2, ...An are the spectrophotometric responses observed at λ1, λ2, ...λn, respectively, and ao, a,, and α1, a2, ...αn-1 are constants determined according to a calibrating method. Such calibrating method is an empirical means for determining and recording in a chemical analyzer the a0,a1, and α1,α2, ...an-i constants essential for making the analyte determination.</p> |
