| 摘要 |
<p>A novel vehicle for cloning DNA in cyanobacteria and other Gram-negative bacteria includes a conjugative replication system and a replication system derived from a suitable cyanobacterial plasmid. The cloning vehicles have enhanced replication function and are able to be introduced into cyanobacterial hosts from other convenient cloning hosts, such as E. coli. The cloning vehicles, while being non-selftransmissible, may be mobilized in the presence of a helper plasmid which provides the necessary transfer functions by trans-complementation. Exogenous DNA may be inserted into the cloning vehicle and conjugally transferred to a target host when the helper plasmid is present on the same or second donor host.</p> |
