摘要 |
PURPOSE:An organ is put into a high-pressure tightly sealable vessel and a preserving solution in which high-pressure oxygen is dissolved is fed to arteries in the organ to enable the long-term storage of the organs with cell tissues kept alive. CONSTITUTION:An excised organ 2 is put into a tightly sealable vessel 1 and the preserving solution in the tank 5g such as Krebs's solution or a physiological saline solution containing nutritions 4 is sent through pipe 5f into the vessel 1 by a certain amount by the pressure of high-pressure oxygen from the bomb 5. Valve 5b is shut, valve 5e is opened to send high-pressure oxygen into the vessel 1 and adjust the pressure inside to 3kg/cm<2> pressure, then the valve 5e is closed. In this state, pump 5b is driven to feed the preserving solution in which a larger amount of oxygen than in air is dissolved 4 which is fed to the arteries 2a of the organ 2 to send an adequate amount of oxygen and nutritions to the tissue cells of the organ and flown out of the cut end of the veins.
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