| 摘要 |
The amount of lipid bound sialic acid in a blood plasma or serum sample may be determined by a method which may be automated involving the following steps: diluting the sample with distilled water; mixing and then cooling the diluted sample; adding a mixture of a chlorinated lower alkyl hydrocarbon and a lower alkyl alcohol; mixing, diluting with water and then treating by mixing further and centrifuging to yield a substantially clear upper phase; recovering the upper phase and adding to it a mixture of a protein precipitating agent and adsorbing material, mixing the resulting admixture; recovering the resulting precipitate, suspending the precipitate in distilled water and determining the amount of lipid bound sialic acid present. |
