发明名称 |
Purified recombinant interleukin-2 and process for recovering and purifying same. |
摘要 |
<p>A process for recovering microbially produced IL-2 in a highly pure form from the cellular material of the microorganism that produced it comprising: disrupting the cell membranes of the microorganisms; extracting the disruptate with a chaotropic agent, such as urea, that selectively extracts microbial proteins from the cellular material; solubilizing the IL-2 in the solid phase of the extraction mixture with an aqueous solution of a solubilizing agent, such as SDS, containing a reducing agent; and separating the IL-2 from the resulting solution by an optional extraction with 2-butanol or 2-methyl-2-butanol followed by gel filtration chromatography, oxidizing the IL-2 and purifying the oxidized IL-2 by RP-HPLC. </p> |
申请公布号 |
EP0156373(A2) |
申请公布日期 |
1985.10.02 |
申请号 |
EP19850103649 |
申请日期 |
1985.03.27 |
申请人 |
CETUS CORPORATION |
发明人 |
KOTHS, KIRSTON EDWARD;THOMSON, JAMES WILLIAM;KUNITANI, MICHAEL;WILSON, KENNETH;HANISCH, WOLFGANG HELMUT |
分类号 |
C12N15/09;A61K38/00;A61P35/00;C07K1/113;C07K1/14;C07K1/20;C07K14/00;C07K14/52;C07K14/54;C07K14/55;C12N15/26;C12P21/00;C12P21/02;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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