| 摘要 |
<p>The present invention provides a process for the production of a micro-organism, wherein not only a DNA containing an .alpha.-galactosidase gene but also an appropriate vector for the transformable cells to be used, which contains antibiotic resistance genes, are completely split with restriction endonuclease Sal I, a fragment is obtained from the fragments of the DNA containing the .alpha.-galactosidase gene which fragment has a relative molecular weight of about 4 megadaltons, this fragment is mixed with a solution of the vector also split with Sal I and recombined in the presence of DNA ligase with the formation of a recombinant DNA, the recombinant DNA obtained is incubated with transformable cells, with transformation of the recombinant DNA in the cells, the transformed cells are cultured on a nutrient substrate containing raffinose as the sole source of carbon, the antibiotic-resistant colonies formed are isolated and lysed, the plasmid DNA is isolated from the lysate, this is split with restriction endonuclease Hind III or Eco R I, the solution obtained is diluted and treated with DNA ligase, the renatured plasmids obtained are again introduced into transformable cells, the transformed cells are again cultured on a nutrient substrate containing raffinose as carbon source, as well as anti biotic, and the colonies farmed which do not utilise raffinose are isolated.</p> |
