| 摘要 |
NEW MATERIAL:A heat-resistant pullulanase. USE:A starch saccharification enzyme such as alpha-amylase: it has a wide range of active or optimally active pH from 4.5-7.5 and shows high heat resistance. PREPARATION:Klebsiella pneumoniae (FERM P-7387), capable of producing heat-resistant pullulanase is cultured in a medium containing nitrogen source such as peptone, carbon source such as starch, phosphate and metal salts such as iron salt, preferably at a pH of 5-9 and 20-45 deg.C under aerobic conditions. |
