| 摘要 |
NEW MATERIAL:Thermoanaerobacter No. 29 (FERM-P 7494) having a plasmid expressed by a restriction enzyme cleavage map and about 3.8 megadaltons molecular weight. USE:Useful as a vector for recombinant DNA experiments using anaerobic thermophiles as a host, having a high biomass conversion efficiency, and capable of breeding the anaerobic thermophiles producing useful chemicals. PREPARATION:For example, hot-spring water of the ATAGAWA spa in SHIZUOKA Preferature is added to a YPG culture medium and cultivated at 70 deg.C for 18hr under anaerobic conditions of gaseous carbon dioxide. Thermoanaerobacter No. 29 is then obtained from one of the colonies grown in a YPG roll tube containing streptomycin. |
