Immunoreactive solid phase.

摘要

A process for activating a solid polymer surface by radiation grafting of vinyl monomers having at least one functional group which can be bound to biologically active molecules, as well as a process for preparing an immunoreactive solid phase wherein said biologically active molecules are covalently bound to said functional groups on the polymer surface. The processes are characterized in that the polymer surface is grafted in solution in a chain transfer reducing solvent at a monomer concentration below 3% by weight when the vinyl monomer is 1-mono-or 1,1-disubstituted, whereby a very thin layer is obtained. The invention also refers to the activated solid phase, obtained by the activating process, for immobilizing biologically active molecules and an immunoreactive solid phase comprising said molecules. The immunoreactive solid phase to which an antigen or an antibody has been covalently bound can be used for quantitative or qualitative determination of antigen or antibody in an immunoassay.