| 摘要 |
<p>Disclosed is a reagent useful in the determination of a component of an antibody-antigen or a receptor-receptor binding molecule reaction. The reagent is characterized by the reaction product selected from the group consisting of: (a) a solid support matrix having the antibody linked thereto and a conjugate of the antigen and a chromagenically responsive marker; (b) a solid support matrix having the antigen linked thereto and a conjugate of the antibody and a chromagonically responsive marker; (c) a solid support matrix having the receptor linked thereto and a conjugate of the receptor binding molecule and a chromagenically responsive marker; and (d) a solid support matrix having the receptor binding molecule linked thereto and a conjugate of the receptor and a chromagenically responsive marker. The reagent is especially useful in the detection of hCG in order to ascertain whether a female is pregnant. For such embodiment, reagent (a) is composed of the reaction product of nylon having anti- beta -hCG linked thereto which is bound to a conjugate of hCG and an enzyme, e.g. horseradish peroxidase, by a carrier molecule such as, for example, Ficoll70 or bovine serum albumin. Various other non-isotopic immunoassay tests are discussed and disclosed for the reagent.</p> |
