发明名称 |
METHOD FOR THE PURIFICATION OF CHOLESTEROL OXIDASE |
摘要 |
A method for the purification and recovery of cholesterol oxidase is disclosed. Cholesterol oxidase is purified by a method including the steps of: (a) contacting a solution of the cholesterol oxidase to be purified with a chromatography composition comprising an unbound water-insoluble steroid which is a nonsubstrate for cholesterol oxidase so as to adsorb the cholesterol oxidase on the steroid; (b) separating the solution from the steroid; and (c) contacting the steroid with an eluting solution containing a surfactant at a concentration between 0.001 percent and 10 percent so as to recover the adsorbed cholesterol oxidase. In a preferred embodiment, the solution containing the cholesterol oxidase to be purified is a microorganism growth medium having cholesterol oxidase activity and, prior to the step of contacting the solution with a steroid, the solution is contacted with an ion-exchange chromatography material. In yet another embodiment, the eluting solution containing the surfactant also contains a metal salt of a concentration of at least about 0.005 molar. There is also disclosed a method for forming a packed bed of a composition comprising the unbound steroid which comprises the step of first suspending the steroid in a water-miscible organic solvent. |
申请公布号 |
DE3265262(D1) |
申请公布日期 |
1985.09.12 |
申请号 |
DE19823265262 |
申请日期 |
1982.10.18 |
申请人 |
EASTMAN KODAK COMPANY |
发明人 |
SNOKE, ROY EUGENE;GOODHUE, CHARLES THOMAS |
分类号 |
C12N9/04;(IPC1-7):C12N9/04 |
主分类号 |
C12N9/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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