Diagnosis of gene abnormalities by restriction mapping using a sandwich hybridization format.

摘要

Assay for detecting the presence or absence of a particular genetic sequence in a nucleic acid strand. Double stranded sample nucleic acid is reacted with a restriction endonuclease whose activity depends on the presence or absence of the particular base sequence to be detected at the restriction site. The DNA is denatured and reacted with an immobilized nucleic acid probe and a labeled nucleic acid probe each of which hybridizes on either side of the restriction site. Separation of the aqueous and solid phases is effected and measurement of the label in either phase related to the presence or absence at the restriction site of the particular base sequence.