| 摘要 |
<p>A method is disclosed for the determination of NAD(P)H which comprises reacting the NAD(P)H with (1) peroxidase or thiol reductase and (2) diaphorase or an electron carrier, in the presence of a chromogen and determining the pigment thus formed. The chromogens used are compounds of the formula(I):
<CHEM>
wherein Y is hydrogen or hydroxyl; R1, R2 and R3 are each
<CHEM>
groups wherein Z is hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl, or alkoxy; n is 0, 1, 2 or 3; provided that at least one Z in R1, R2 and R3 is hydroxyl, amino, or substituted amino.
<??>Also disclosed are reagents comprising the chromogen in admixture with the peroxidase or thiol oxide reductase and the diaphorase or electron carrier. </p> |
