| 摘要 |
A method of quantitatively determining the level of hemoglobin in a biological material which includes the steps of preparing a test sample of the biological material, converting the heme portion of the hemoglobin in the test sample to porphyrin, assaying the fluorescence of the converted porphyrin and comparing the fluorescence of the converted porphyrin to a standard. The invention also relates to an apparatus for performing the above method and a sampler device for collecting and preparing a test sample of the biological material, wherein the apparatus includes at least two reaction chambers with one chamber containing oxalic acid and ferrous oxalate for converting the heme portion of hemoglobin in a test sample to porphyrin and the other chamber containing citric acid which is generally non-reactive with the heme portion of hemoglobin to function as a control blank for the hemoglobin determination.
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