| 摘要 |
A detectable molecule of the formula A<3>-(-X-R<1>-E-Det<b>)m where A<3> is A<2> or a polymer, where A<3> has at least one modifiable reactive group selected from the group consisting of amino, hydroxy, cis OH, halides, aryl, imidazoyl, carbonyl, carboxy, thiol or a residue comprising an activated carbon; -X- is selected from the group consisting of <CHEM> -N=N-, -NHSO2-, -OSO2-, -NH-N=N-, <CHEM> -CH2-NH-, <CHEM> -NH-CH2-, <CHEM> -S-CH2-, -O-CO-NH- -R<1>- is <CHEM> or a C1-C10 branched or unbranched alkyl or aralkyl, which may be substituted by -OH; -Y- is a direct bond to -E-, or -Y- is -E-R<2>- where R<2> is a C1-C10 branched or unbranched alkyl; Za is chlorine, bromine or iodine; E is O, NH or an acyclic divalent sulfur atom; Det<b> is a chemical moiety capable of being detected, preferably comprising biotin or a metal chelator of the formula: <CHEM> or the 4-hydroxy or acyloxy derivative thereof, where R<3> is C1-C4 alkyl or CH2COOM, M is the same or different and selected from the group consisting of hydrogen, a metal or non-metal cation or is C1-C10 alkyl, aryl or aralkyl; and m is an integer from 1 to the total number of modified reactive groups on A<3>. The detectable molecules are useful in in vitro or in vivo assays or therapy. |
