ELECTROCHEMICAL DETERMINATION OF ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE ACTIVITY (EC 3.1.1.1 AND EC 3.1.3.2: ALKALINE AND ACID PHOSPHATASES)

摘要

ELECTROCHEMICAL DETERMINATION OF ORTHOPHOSPHORIC MONOESTER PHOSPHOHYDROLASE ACTIVITY (EC 3.1.3.1 AND EC 3.1.3.2: ALKALINE AND ACID PHOSPHATASES) A method is disclosed for the measurement of phosphate ester substrate containing a nitro group is reacted with acid or alkaline phosphatase under appropriate reaction conditions and the hydrolytic process is monitored at electrodes which measure the current produced by the reduction of the nitro groups of the product and/or substrate. Alternatively, an aromatic phosphate ester substrate containing an amino group is reacted with acid or alkaline phosphatase under appropriate reaction conditions with the hydrolytic process being monitored at solid electrode which measure the current produced by the oxidation of the amino groups of the product and/or substrate.