| 摘要 |
PURPOSE:To obtain human urogastrone (human UG for short) having high accuracy in high yield, by collecting a fraction containing human UG from a test specimen containing human UG by liquid chromatography of reverse phase distribution type. CONSTITUTION:A test specimen (e.g., urine itself, filtrate obtained by filtering urine with Celite, extracted solution from a tissue or cell producing human UG, etc.) containing human UG is subjected to liquid chromatography of reverse phase distribution type. In the operation, porous styrene-divinylbenzene copolymer particles, etc. are used as a column agent to be packed into a column of reverse phase distribution type. Then, a fraction containing human UG is collected. The fraction is concentration under reduced pressure to distill an organic solvent away, it is desalted by gel filtration, etc., and lyophilized, to give human UG. USE:Having an inhibitory action on secretion of acid in the stomach, and promoting action on growth of epithelical cell and other cells, useful as a drug or an addition agent used in tissue culture.
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