| 摘要 |
PURPOSE:To make it possible to prepare a vector having a multiple promoter, by using a vector having a promoter range between scission sites of two restricted enzymes to form the same attachment site. CONSTITUTION:Scission site Hind III of plasmid pDR540 is changed into scission site BglII. Namely, pDR540 is digested with Hind III by a conventional procedure, extracted with phenol chloroform, and precipitated with ethanol. The precipitate is dissolved in sterilized water, and BglIII linker is subjected to ligation by a conventional procedure. It is digested with BglII, the prepared reaction solution is subjected to electrophoresis with 1wt% agarose, and DNA band of about 4,000pb is recovered by electroelution. This DNA fragment is subjected to ligation by a conventional procedure. In this way, plasmid pTL1 wherein scission site Hind III of pDR540 is replaced with scission site BglII is obtained. This pTL1 has BglII and BamH, and has a tac promoter between them.
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