摘要 |
<p>NEW MATERIAL:Monoclonal antibody 21-5-12 against human phenylalanine hydroxylase, secreted from hybridoma cell line 21-5-12. USE:A reagent useful for detection of human phenylalanine hydroxylase and other antigens capable of reacting with a monoclonal antibody by immune fluorescent microscopy, etc., for purification (isolation) and determination of the enzyme, especially for diagnosis, remedy, etc. of phenylketonuria. PREPARATION:Hybridoam cell line 21-5-12 is cultivated, and a yielded antibody is collected to obtain a monoclonal antibody 21-5-12 against human phenylalanine hydroxylase. The antibody is linked to a solid phase, a biological test specimen is added to it, the enzyme in the test specimen is linked to the antibody under heating. The linked material is washed, the enzyme is separated from the solid phase, and the enzyme is simply isolated from the test specimen. Further, the enzyme can be determined by using the isolated enzyme and the antibody labeled with radiation or an enzyme.</p> |