发明名称 |
Characterization of oncogenes and assays based thereon |
摘要 |
Experiments designed to define the differences between an oncogene isolated from human bladder cancer cells and its corresponding proto-oncogene are described herein. By a series of in vitro recombinations, the difference was initially isolated to a 350 kb segment of DNA; sequencing defined the difference as a change in the Gly12 codon causing the p21 protein of the oncogene to contain valine at a location where the p21 protein of the proto-oncogene contained glycine. Assays for detecting carcinogenesis based on such differences are also described. In one type of assay, a restriction enzyme specific for either the altered or non-altered DNA segment of the genes are employed to detect carcinogenesis. In another type of assay, seralogical reagents, such as antibody specific for either p21 protein expressed from the proto-oncogene or oncogene, or a common site therein, are described.
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申请公布号 |
US4535058(A) |
申请公布日期 |
1985.08.13 |
申请号 |
US19820432337 |
申请日期 |
1982.10.01 |
申请人 |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
发明人 |
WEINBERG, ROBERT A.;TABIN, CLIFFORD J.;BRADLEY, SCOTT M. |
分类号 |
A61K39/395;A61K39/00;C07H21/04;C07K14/435;C07K16/00;C07K16/30;C07K16/32;C07K19/00;C12N15/00;C12N15/09;C12P21/00;C12P21/08;C12Q1/68;G01N33/50;G01N33/53;G01N33/531;G01N33/574;G01N33/577;(IPC1-7):C12Q1/68;C12Q1/48;C12Q1/34;C12P19/34;G01N15/02;A61K43/00 |
主分类号 |
A61K39/395 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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