发明名称 |
HOMOGENEOUS NUCLEIC ACID HYBRIDIZATION DIAGNOSTICS BY NON-RADIATIVE ENERGY TRANSFER |
摘要 |
<p>A homogeneous light-emitting hybridization assay is disclosed wherein light-labeled first and second single-stranded reagent segments are hybridized with a complimentary target singlestranded polynucleotide from a physiological sample such that non-radiative energy transfer occurs between the light labels of the two reagent segments. At least one of the light labels is of the absorber/emitter type such that energy absorbed from the other light label is re-emitted as a different wavelength. Such secondary emissions can only occur if hybridization has taken place and hence the presence of the target polynucleotide is related to the amount of secondary light emitted.</p> |
申请公布号 |
CA1190838(A) |
申请公布日期 |
1985.07.23 |
申请号 |
CA19820406767 |
申请日期 |
1982.07.07 |
申请人 |
STANDARD OIL COMPANY |
发明人 |
HELLER, MICHAEL J.;MORRISON, LARRY E.;PREVATT, WILLIAM D.;AKIN, CAVIT |
分类号 |
G01N33/50;C07H21/04;C12N15/00;C12Q1/28;C12Q1/66;C12Q1/68;(IPC1-7):G01N33/58 |
主分类号 |
G01N33/50 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|