| 摘要 |
Centrifugation apparatus, especially for cytocentrifugation to produce cell monolayers, includes a sample chamber that is adapted for automated protective processing, e.g. fixation, of matter deposited on a slide during the centrifugation operation. For this purpose the sample chamber comprises a deposition chamber (5), and a treatment fluid reservoir (9) communicating with the deposition chamber (5) via a buffer chamber (10) and weir (11) so arranged that in the attitude for centrifugation, treatment fluid such as fixative placed in the reservoir (9) is retained there until centrifugation produces an artificial gravitational field that causes the fluid to flow to the buffer chamber (10) in which it is retained by the weir (11) for so long as the artificial gravitational field is maintained. Upon decay of that field the fluid flows over the weir (11) to the deposition chamber. There may be a series of such reservoirs and associated buffer chambers and weirs through which buffer chambers a succession of treatment fluids are caused to progress in sequence by repeatedly applying the artificial gravitational field. There may also be a sample reservoir (7) that communicates with the deposition chamber (5) in a manner providing for transfer of sample material from this reservoir (7) to the deposition chamber (5) only when the artificial gravitational field is applied. |
