Polysaccharide PS-A obtained from barrenwort deriving from plants belonging to the genus Epimedium, process for preparation thereof and phylactic and immunostimulating agents comprising said polysaccharide PS-A effective component

摘要

PCT No. PCT/JP82/00132 Sec. 371 Date Dec. 28, 1982 Sec. 102(e) Date Dec. 28, 1982 PCT Filed Apr. 20, 1982 PCT Pub. No. WO82/03771 PCT Pub. Date Nov. 11, 1982.A polysaccharide PS-A obtained from plants belonging to the genus Epimedium and having the following physicochemical properties, a process for preparation thereof and use as phylactic and immunostimulating agents comprising the polysaccharide PS-A as an effective component: 1. Elementary analysis: C=40.92; H=6.17; Ash=very small; 2. Molecular weight: 75,000+/-25,000 (average molecular weight); 3. Decomposition point: 205 DEG C.; 4. pH: 7.0 (solution of 100 mg of PS-A in 50 ml of water); 5. Specific rotatory power: [ alpha ]D19=-23.6 DEG (in H2O, c=0.527); 6. Infrared absorption spectrum: nu maxKBr(cm-1)/3400, 2900, 1620, 1400, 1230, 1060; 7. Ultraviolet absorption spectrum: Maximum absorption is not recognized in the range of 240-400 nm.; 8. Outward form: White or faint brown, amorphous powder; 9. Solubility: (a) Soluble in water; (b) Insoluble in methanol, ethanol, acetone, ethyl acetate, diethyl ether, hexane and chloroform; 10. Color reactions: Positive to the following reactions: (a) anthrone-sulfuric acid reaction; (b) Molisch's reaction; (c) skatol reaction; and (d) Bial's reaction; Negative to the following reactions: (a) ninhydrin reaction; (b) 2,4-DNP reaction; (c) Selivanoff's reaction; (d) naphthoresorcinol reaction; and (e) carbazol-sulfuric acid reaction; 11. Component sugars: Arabinose and galactose; 12. Homogeneity: Homogeneity is proved according to ultracentrifugation, electrophoresis and gel filtration.