| 摘要 |
PURPOSE:To promote the production of beta-amylase, by culturing a beta-amylase- producing bacterial strain in a medium containing thioglycolic acid (salt). CONSTITUTION:A beta-amylase-producing strain belonging to Bacillus genus (e.g. Bacillus sereus IFO-3001) is cultured in a synthetic medium or natural medium containing about 0.0001-0.05%(W/V) thioglycolic acid or its salt (e.g. sodium salt, ammonium salt, etc.) at about 25-35 deg.C and about 7.5-9pH for about 50- 70hr under aerobic condition, and the objective beta-amylase is separated from the resultant cultured product. The thioglycolic acid (salt) is added to the medium preferably when the production of beta-amylase begins after the proliferation of the bacterial cells. |
