| 摘要 |
PURPOSE:To obtain a novel polypeptide having human IL-2 activity, by preparing a DNA having a specific base sequence or a transformation containing said DNA. CONSTITUTION:The fraction of the human IL-2 gene of a plasmid containing human IL-2 gene is incised with a restriction enzyme, and separated e.g. by electrophoresis. The obtained DNA is dissolved in an SI buffer solution, and treated at room temperature for 0.5-3min in the presence of 0.2-0.8 unit of SI unclease to obtain the DNA having the base sequence of formula wherein V is hydrogen or codon other than AGC, AGT, TCA, TCC, TCG, TCT, TAA, TAG, and TGA, and Y is codon or hydroxyl group. |
