| 摘要 |
PURPOSE:To prepare a peculiar antibody to vanylmanderic acid, by a method wherein bovine serum albumin is chemically bonded to vanylmanderic acid to impart immunogenicity thereto and, after an animal is innoculated with the resulting conjugate, blood is collected from said animal and the specific antibody is separated from the blood and purified. CONSTITUTION:Vanylmanderic acid is dissolved in a saline solution and 1-ethyl- 3, 3-dimethylaminopropyl hydrochloride and bovine serum albumin are added to said solution. This reaction solution is applied to column chromatography using Sephadex G-25 to separate a conjugate of vanylmanderic acid and bovine serum albumin. In the next step, a rabbit is hypodermically innoculated with the resulting conjugate as a protein concn. along with a Freund's complete adjuvant and, thereafter, blood is collected from the rabbit. Whereupon, the production of a peculiar antibody to vanylmanderic acid is discovered in the serum of the blood. This specific antibody is separated and purified by affinity chromatography prepared by coupling AH-Cephalose 4B with vanylmanderic acid. |
