| 摘要 |
PURPOSE:To provide the titled cloned DNA which is very useful as a probe or primer in the cloning of a factor nocrosing cancer in different animals including human and is used to form a microorganism or cells for producing the necrosing factor. CONSTITUTION:The DNA which is represented by the formula and has base sequence that can code the polypeptide containing the aminoacid sequence of the factor necrosing rabbit cancer. At first, the cancer-necrosing factor produced in the rabbit body is isolated and purified to clarify the chemical properties of the protein, then the mRNA of the factor is efficiently produced in vitro using macrophage of rabbit albeoruses and the product is isolated and concentrated. Then, gene recombination is effected to clone cDNA coding the factor necrosing rabbit cancer. |
