Method for preparing a cell free homogenate of Chrysanthemum cinerariaefolium (Trev.) Bocc. containing enzymes and methods of use

摘要

A method is disclosed for preparing a cell free homogenate from Chrysanthemum species and Tagetes species of the family Compositae which contains the bioactive enzyme(s) and cofactor(s) of the pyrethrum pathway. A method is also disclosed for preparing a cell free matrix containing these bioactive enzyme(s) and cofactor(s). The homogenate and matrix will convert mevalonic acid, labeled mevalonic acid, isopentenyl pyrophosphate or labeled isopentenyl pyrophosphate into pyrethrins, labeled pyrethrins, chrysanthemyl alcohol or labeled chrysanthemyl alcohol.