Method of assay of DNA or RNA from virus particles.

摘要

In the extraction of DNA or RNA from virus particles particularly for assay of viral DNA or RNA, e.g. for hepatitis B virus, it has been a problem that the normal procedure involves precipitating the virus by centrifuging the sample, followed by extracting into phenol, which is unpleasant to handle, and the addition of large amounts of expensive protease enzyme to remove the proteolysis products. In the invention, these problems are avoided by a method which comprises precipitating virus particles from the sample by incubating it with an insolubilised monospecific, preferably monoclonal antibody to a surface antigen of the virus, breaking open the capsid of the precipitated virus particles and releasing the viral DNA or RNA thereof, extracting the viral DNA or RNA into the liquid phase, and separating the solid and liquid phases. Very desirably the above method of extraction of viral DNA or RNA is combined with a two-site hybridisation assay to provide a very convenient and sensitive assay for viral DNA or RNA. A kit for use in connection with this method comprises the monospecific antibody and the two polynucleotides, not necessarily in double-stranded ds-form, and the first not necessarily insolubilised.