AFFINITY RESINS AND THEIR USE IN ISOLATING BACTERIAL LUCIFERASE

摘要

Novel affinity resins can be used for the isolation of bacterial luciferase, by saturating the resin with a sample containing bacterial luciferase suspended in an anionic buffer solution; washing the saturated resin with additional anionic buffer to remove unbound protein; eluting the washed saturated resin with a cationic buffer solution; and recovering the bacterial luciferase. Novel resins which can be used for this purpose comprise a support material, a spacer arm and a ligand, and are represented by formulae J, K and M.