| 摘要 |
<p>A device for performing qualitative enzyme immunoassays comprises at least one tube (11) having antibody, antigen or hapten attached to an internal surface thereof. A first syringe (17) is connectable to a first end of the tube or tubes (11) and supplies a flow of test liquid therethrough. A second syringe (18) having a two piston arrangement (33, 35) is connected to the tube so as to allow a first wash solution followed by an enzyme conjugate solution to be flowed therethrough. Finally a third syringe (19), also having a two piston configuration (33, 35), is connected to the tube (11) so as to allow a second wash solution followed by an enzyme substrate and metabolite indicator solution to be flowed therethrough. The device can be used in a method in which the test liquid is flowed through the tube for about 1 minute, the first wash solution is then flowed through the tube followed by an enzyme conjugate solution, followed after an incubation period of at least one minute by a second wash solution and a solution containing enzyme substrate and enzyme metabolite indicator.</p> |
