| 摘要 |
A proteinaceous substance having the following biochemical characteristics: (A) a strong cytolytic activity against mouse tumor cells L-929, but not cytotoxic activity for normal cells such as human fetus fibroblasts, human adult skin fibroblasts and chinese hamster fibroblasts (Don, V-79) and therefore a high tumor specificity: and (B) a strong anti-tumor and immunogenic activity against Meth-A sarcoma. When said substance is injected intravenously to the syngenic BALB/C mice inoculated with Meth-A sarcoma at a quantity of more than 72 units head, not only hemorrhagic necrosis, but also complete degradation is observed. Thereafter, even if it is attempted again to transplant the Meth-A sarcoma cells under the skin of Mice cured completely, successful plantation is not observed because of an acquired immunity: (C) the substance has no pyrogenetic action after intravenous injection to rabbits: (D) a molecular weight of 45,000+/-5,000 (determined by the gel filtration method with Sephacryl S-200): and (E) an isoelectric point pI of 4.8+0.3: and a method for its manufacture. |
