Microbial process for producing 12 alpha -hydroxypregna-1,4-dien-3-one-20 alpha -carboxylic acid

摘要

A microbial process for producing 12 alpha -hydroxypregna-1,4-dien-3-one-20 alpha -carboxylic acid or a salt thereof which comprises cultivating a microbe of the species Pseudomonas arvilla or the genus Alcaligenes, e.g. Alcaligenes faecalis, which is capable of producing 12 alpha -hydroxypregna-1,4-dien-3-one-20 alpha -carboxylic acid or a salt thereof by utilizing deoxycholic acid or a salt thereof as a substrate, in a culture medium containing the substrate and collecting the resulting compound.