PROCEDIMENTO PER IL TRATTAMENTO DI ERITROCITI PER LA PREPARAZIONE DI MATERIALI DI SAGGIO E PRODOTTO OTTENUTO

摘要

A double aldehyde treatment process of erythrocytes is disclosed. Erythrocytes from various sources can be treated in a two-step process to render them stable and subsequently useful in antigen antibody detection systems. Glyoxal is used as a first treating medium followed by a second treatment step using formaldehyde or glyoxal as amounts ranging from 0.1 to 0.4 gm. per 0.8 ml. of Packed Cell Volume of erythrocytes, followed by the second treatment step in which at least 0.1 gm. of formaldehyde or glyoxal and preferably 0.1 to 0.6 gm. per 0.8 ml. Packed Cell Volume of treated erythrocytes is used. The reaction medium is preferably aqueous hypertonic and most preferably a sodium citrate medium. The treated cells can be used in detection of hepatitis associated antigen in a reverse passive hemagglutination test.