ANTI-HLA-A2 ANTIBODY AND HYBRIDOMA PRODUCING THE SAME

摘要

PURPOSE:To provide a monoclonal antibody FLA02 having high antibody value, and produced by the fused cell obtained from the spleen cell of mouse immunized with human B lymphoblastoid cell having human HLA-A2 antigen. CONSTITUTION:An Sh cell which is human B lymphocyte cell having HLA-A2 antigen and HLA-A9 antigen in a heterogeneous form is infected with EB virus. The obtained lymphoblastoid cell is proliferated and immunized to a mouse as an immunogen. The spleen cell collected from the mouse is fused with mouse myeloma cell, and the resultant fused cell is cultured, from which hybridoma SH-2-119-6 having the highest cytotoxicity to Sh cell is separated. The objective monoclonal antibody FLA-2 having complement-dependent cytotoxicity and reactive specifically with HLA-A2 antigen is obtained from the supernatant liquid prepared by the cultivation of the hybridoma in a proper medium, or from the ascites obtained by the peritoneal infusion of the hybridoma to a mouse.