发明名称 |
METHOD FOR THE PURIFICATION AND ISOLATION OF BLOOD CLOTTING PROTEINS USING CONFORMATION SPECIFIC ANTIBODIES |
摘要 |
A method of isolating a protein from a mixture containing the protein, the method including providing an antibody immobilized on a solid support, which antibody is reactive with the protein complexed with a ligand and substantially unreactive with the protein not complexed with the ligand; contacting the mixture, in the presence of the ligand, with the immobilized antibody to bind the protein, complexed with the ligand, to the immobilized antibody to form an immune complex; and contacting the immune complex with a compound having a binding affinity for the ligand higher than the binding affinity of the protein for the ligand, to release the protein from the immobilized antibody. |
申请公布号 |
WO8501941(A1) |
申请公布日期 |
1985.05.09 |
申请号 |
WO1984US01746 |
申请日期 |
1984.10.29 |
申请人 |
NEW ENGLAND MEDICAL CENTER HOSPITALS, INC. |
发明人 |
FURIE, BRUCE, E.;FURIE, BARBARA, C.;LIEBMAN, HOWARD, A.;LEWIS, RICHARD, M. |
分类号 |
A61K35/16;A61K38/00;C07K1/14;C07K1/22;C07K14/745;C07K14/755;C07K14/76;C07K16/00;C07K16/36;C07K16/40;C07K17/00;C12N9/00;C12N9/64;C12N9/74;C12P21/08;C12R1/91;G01N33/53;G01N33/531;G01N33/543;(IPC1-7):C07G7/00;A61K39/395;C07G7/04 |
主分类号 |
A61K35/16 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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