| 摘要 |
PURPOSE:To enable quick quantitative analysis of an antigen and antibody by measuring the chemically emitted light or biologically emitted light occuring in the material contributing to light emission in the microcapsule released from the inside of the microcapsule by breakdown owing to antigen antibody reaction and more in amt. than the antibody or antigen. CONSTITUTION:A microcapsule in which a material contributing to light emission is sealed, a complement which breaks down the microcapsule film by acting the antigen- antibody composite body generated by the conjugation of the antigen and the antibody and a material which induces light emission by reacting with the material contributing to light emission are prepd. The microcapsule and the antigen or antibody which is the material to be measured as well as the complement are mixed. The antigen-antibody reaction is then induced on the microcapsule film to form the antigen-antibody composite body. The complement is acted with such antigen-antibody complex to dissolve or break down the microcapsule film so that the material contributing to light emission sealed therein flows out into the system. The material which induces the light emission by reacting with the material contributing to light emission is added to the system having the outflow material contributing to light emission in the amt. larger than the number of molecules of the antigen or antibody, by which the light emission is induced and the material contributing to light emission is quantitatively determined. The material to be measured is thus analyzed. |
