| 摘要 |
PURPOSE:To stabilize an enzyme and to enable the repeating use of it, by immobilizing an enzyme to hydrolyze a specific oxazolidine to a hydrophobic carrier. CONSTITUTION:An esterase capable of asymmetrically hydrolyzing (A) a racemic modification of 3-alkyl-substituted-5-acyloxymethyloxazolidin-2-one shown by the formula I (R1 is 1-4C alkyl group; R2 is alkyl group) is immobilized to a hydrophilic carrier, and the prepared immobilized enzyme is packed into a column. The compound A is loaded onto the column, and water or a buffer solution is made to flow into it, to carry out asymmetric hydrolysis. An optically active hydrophilic 3-alkyl-substituted-5-hydromethyloxazolidin-2-one shown by the formula II prepared by the reaction is eluted with water or a buffer solution. Then, an optically active hydrophobic 3-alkyl-substituted-5-acyloxymethyoxazolidin-2- one shown by the formula III which is adsorbed on the carrier of the immobilized enzyme in the column, supported, and is not a substrate of the asymmetric hydrolysis, is diluted with an organic solvent. |
